Honey Quality – How to Check for Honey Adulteration Without Lab Testing

honey

The top google results for "check fake honey" are all nonsense blogs that say to check based on water content. The problem with this is that real honey can have a higher water content, especially here in asia. So is there a way for me to tell if a non-viscous honey has added HFCS or something similar in it?

I am considering dissolve rate as a possible option because real honey should have more longer carbohydrates…

I found a lab technique using carbon 13 isotopes.http://www.iso-analytical.co.uk/honey.html but i doubt there are DIY kits on the web for this.

Best Answer

The answer, quite simply, is no. Even advanced laboratories have difficulty making the determination for certain.

This is also a complex problem that gets more difficult as various standards are used and then fail. Before 2000, a common solution was to simply use microscopic analysis to look for pollen and and other vegetable matter. Since then, many honey processing plants have been developing increasingly advanced filtering techniques that will remove the characteristic markers (deliberately or unintentionally). [**See detailed note below.] Various chemical or basic physical markers also have proved insufficient, since the sugar composition of honey can be faked quite well with various sugar syrup blends.

The accepted standard these days, as mentioned in the question, seems to be to use a mass spectrometer to determine the carbon-13 to carbon-12 isotope ratio in a rather specific lab procedure. (Obviously most people don't have a mass spectrometer at home.) The current procedure for this test was adopted after previous lab tests were shown to generate false positives in some batches of honey. The isotope ratio method is the only one specifically listed in the FDA's import alert to determine the possibility of adulteration:

FDA laboratories do not have the instrumental capability to analyze honey according to the Official Methods of Analysis of AOAC International, AOAC Official Method 991.41, which requires an isotope ratio mass spectrometer.

Ironically, in order to avoid the previous false positives for the New Zealand honey mentioned above, the new testing process needs to have the pollen completely removed, a process which has also been used to hide the origin of honey and to confuse analysis:

To eliminate a false positive C(4) sugar test for Manuka honey, prior removal of pollen and other insoluble material from the honey is necessary to ensure that only the pure protein is isolated.

But even a refined isotopic methodology is flawed when it comes to detecting various types of adulteration, specifically beet sugar. As this article notes:

[Using isotope ratios from a mass spectrometer,] adulteration using C4 sugar syrups (HFCS and GS) could be detected to a certain extent while adulteration of honey using C3 sugar syrups (beet sugar) could not be detected. Adulteration by using SS (beet sugar) still has a serious detection problem, especially in countries in which beet is used in manufacturing sugar.

So, what's the alternative? Well, the other general method that could detect various adulterating components is differential scanning calorimetry (DSC). This article gives a good summary of the process, which essentially looks at how a material behaves as it undergoes thermal changes. At certain temperatures when crystallization or something occurs, there will be excess heat absorbed or given off compared to at other temperatures. And at other points there will be minor changes in heat capacity (i.e., the amount of heat it takes to alter the temperature of a substance by a specific number of degrees).

Honey, for example, displays a glass transition temperature (Tg) around -40°C (-40°F) near a certain point in crystallization. Other sugar syrups may not show this, but they may show changes at slightly higher temperatures (still below freezing), due to water crystals freezing or thawing. (Water is included in the sugar network in honey, so it doesn't show the same characteristics.)

There are other thermal properties that can be measured at various temperatures. As this article summarizes in its conclusion:

Used concomitantly with the second enthalpy of fusion (occurring between 40 and 90°C), the glass transition temperature, Tg, is one of the most potentially useful parameters for characterizing honeys and syrups and for distinguishing between them. The Tg value, being strongly dependent on the amorphous phases of the sample, will respond to modification of the chemical composition and the implicit structural modification caused by the addition of exogenous material. Thus, adulteration of the honey will cause inevitable changes in both Tg and [delta]H2 values. Under laboratory conditions, adulterations by industrial sugar syrups can be detected from 5-10% additions depending on the measured parameter.

I would pay particular attention to this final sentence -- the differences can only be detected "under laboratory conditions" where precise temperatures and amounts of heat can be measured. To replicate such a test at home, you'd need to be able to add a certain precise amount of heat to the honey at subzero temperatures, all the while keeping it isolated from other sources of temperature fluctuations and observing where the heating "stalls" briefly. Then, you'd have to calibrate your homemade test against some known samples (syrups, 100% honey, etc.) just to be sure you're actually observing the same things as in the article cited here. You'd need to confirm that by observing a more subtle difference in heat capacity changes that would occur in hot temperature ranges (below boiling).

Even under lab conditions, this sort of testing has a threshold of 5-10% adulteration, and that requires something like being able to detect the difference between a glass transition onset at -41°C vs. -42°C. Also, it should be noted that these physical characteristics are inconsistent among various batches of honey. In this study, for example, the Tg was found to have a variance of over 7°C in different pure honey samples. In the study quoted above, that range of 7°C would indicate a difference between pure honey and a 50/50 mixture with a sugar solution. (If you look around at other studies, like this one and this one, you begin to see a Tg range that is well over 15°C for various pure honey types.)

I'd guess this is partly the reason why DSC isn't generally adopted as an official testing procedure: to use it effectively, you'd need to really know the specific kind of honey you started with before blending with adulterants, and most of the time you don't.

Bottom line: there's just no way to do a test like this at home.

Finally, to address a point raised in the question, based on the DSC data there should be minor differences in honey's behavior at various temperatures, perhaps even how fast it dissolves at a certain temperature. But the differences are so small and/or inconsistent among different types of honey or different types of adulterating components that there's no practical way of consistently identifying them outside of a lab environment where very precise conditions and measurements are possible. It might be possible to isolate adulterated samples outside of a lab given prior knowledge of the original honey used and the specific adulterants that might be present, but that information is generally not available. If it were a simple matter of a test like "let's mix this honey in some water and measure how long it takes to dissolve," government regulations would not be resorting to mass spectrometers to try to detect adulteration.


Note that this answer really only "scratches the surface" of the various testing methods available. Here's a partial list of possible tests. Even a cursory search will uncover hundreds of scientific articles describing the advantages and limitations of various tests. Note that most of the other tests only detect specific kinds of adulteration and/or are mostly used as initial screening tests that then need to be verified by another lab procedure. As mentioned, the current standard seems to be an isotope ratio test.


** ADDED CLARIFICATION ON POLLEN AND FILTRATION: Some pollen is generally removed in the normal filtration process used to produce a "clear" honey that doesn't crystallize quickly during storage. However, traditional filtration techniques often allow trace amounts of pollen to remain, while some processes may use a more complex "ultrafiltration" method that will remove all pollen traces. The reason for complete pollen filtration may have originated with a desire to disguise the geographic origin of honey, whether pure or adulterated. In 2001, for example, the U.S. instituted high tariffs on Chinese honey, to avoid putting American beekeepers out of business. At other times, various countries have instituted outright bans on honey for periods due to contamination or adulteration, such as the EU ban of Indian honey in 2011-12. Such actions have provided strong incentives for Asian honey producers to disguise the origin of honey, even if it is unadulterated. The result is that large amounts of commercially available honey are now filtered to remove all pollen, which has a side effect of making adulteration detection much more complex. That said, it should be noted that normal filtration may also result in very low or undetectable amounts of pollen, so the absence of pollen is not necessarily evidence that any deception is intended. (See further details and explanation here.) However, processing methods that deliberately remove all pollen have been used by those who wish to disguise origin and/or adulterate honey with cheaper substitutes. The question specifically inquired about Asian honeys which had been diluted with water; given that ultrafiltration often involves adding water during processing and has apparently been used by some Asian producers, I originally wrote my answer to target the specific honey type inquired about. Once again: an undetectable level of pollen in other countries and from other producers is NOT necessarily evidence of anything nefarious.