Mold goes away when the water goes away, so unless your toilet is leaking again, you have some other source of water or leak on the upper floor. This could be any number of things, even things not plumbing related, like water getting behind the siding, or a leaking roof with water running down the inside walls. As far as killing the mold that is there, I wouldn't waste my time. If you don't correct the water problem then the mold will return very shortly.
I need to figure out if there's still a leak or some kind of humidity problem. Is it safe to assume that because the problem got worse there is? Should I try to find the pipes and see if they're leaking?
Since you have had issues with the toilet in the past that would be the first area I would check. If the drain pipes for your upper floor are leaking then I would expect to see discoloration bleeding through the paint on the ceiling and the walls. If this turns up nothing then in the hole you have already cut, I would get a professional moisture detector device like the one a plumber would use.
These can help detect moisture and humidity in the air which can give you clues if you found the potential problem areas. Don't buy a cheap one because they don't work very well, I would instead see if a rental place will lend you a good one.
I think I'm seeing some black wood in there (need a better flashlight!).
This doesn't necessarily mean anything, it could be discoloration from previous water damage.
I would still like to hire someone to fix the plaster ceiling. Is it going to cost a huge amount extra if I cut through the mesh and they have to replace some of it? Does area make a massive difference in price for that kind of job? For what it's worth, I'm fine having drywall put up instead of plaster.
How big is the hole that you had cut? You can sometimes repair a hole by cutting a small piece of drywall and fitting that back in place, then using some thinset or spackle around the edges. Sand and repeat thinset or spackle two or three times until you have a smooth paintable surface.
Best Answer
Thermal insulation (and a vapor barrier) is applied between the interior and exterior surfaces of the home. Unless you plan on leaving your basement unheated, you wouldn't place thermal insulation between the floors. And leaving it completely unheated is a bad idea since pipes can freeze.
The only reasons to place insulation between floors is for sound or to slow the spread of fire. For fire protection, the places you'd install insulation are where fire could spread up an opening like a chase for running duct work and other utility lines.
What's left is sound protection, and that is best provided by using flooring materials like carpeting above, and then separating the drywall from the joists with something like resilient channel. Insulation in the ceiling cavity will only reduce the high frequency sounds from above. The low frequency sounds, such as loud footsteps, will travel directly through the flooring, the joists, and be rebroadcast by the drywall. Think of sound like electricity, the joists as a short, and carpeting and resilient channel as tools to prevent that short.