Wood – Load wood beam support

I'd go with @Aarthi's load bearing table resource for a general idea of what's reasonable.

If you're looking for equations though, you can start with these:

Beam Deflection Formulas

Beam Deflection and Stress Calculator

Area Moments of Inertia

Using the Parallel Axis Theorem

Wood Material Properties (Modulus of Elasticity (E) found in Table 4-3a)

For the dynamic loading, you'll want to do something similar to the fun I had on this question.

...and you may want to consult a good Mechanics of Materials book. (cheaper paperback international edition on Ebay)

As @Ian points out, the problem is not a simple one and is best solved by simply using what's worked for other people in the past. Go take a look at the swings at your local park and use the same size of beam, provided the span is comparable.

Also, if you're really worried, you could always make the rope into a 'Y' to eliminate bending stress on the beam, leaving it solely in shear. This way, the beam is bearing the compression load from the lateral tension on the 'Y', which will keep the trees from bowing toward each other.

Diagram:

|      |______________________|      |
|      |  |                 | |      |
| tree |  |                 | | tree |
|      |__|_________________|_|      |
|      |   \               /  |      |
|      |    \             /   |      |
|      |     \           /    |      |
|      |      \         /     |      |
|      |       \       /      |      |
|      |        \     /       |      |
|      |         \   /        |      |
|      |          \ /         |      |
|      |           Y          |      |
|      |           |          |      |
|      |           |          |      |
|      |           |          |      |
      ...more rope and trees...
|      |           |          |      |
|      |           |          |      |
|      |         -----        |      |
|      |       /  ___  \      |      |
|      |      |  /   \  |     |      |
|      |      |  \___/  |     |      |
|      |       \       /      |      |
|      |         -----        |      |

There are lots of span calculators available online, which help you determine what size lumber to use in home or deck construction. For example

http://www.awc.org/codes-standards/calculators-software/reversecalc

and

http://www.awc.org/codes-standards/calculators-software/spancalc

You could try to figure out what the live loads and dead loads for the bed are and go from there.

Shortcut - I might try to get by with 2x4's spaced 2' or less apart if they run side to side across a single mattress, but I'd want 2x6's if they run long ways, or for a full or larger.