Ether – How to Make Batch Transaction in Ethereum Using Web3.py

Your nonce is going to be absurdly high. The nonce for an account starts at 0 and increases with each outgoing transaction. You can get the current correct nonce via web3.eth.getTransactionCount.

Your gas limit is also quite high: 2.5 million. At 60 gwei and recent ether prices, consuming that much gas would cost a couple hundred US dollars. Now, your transaction probably won't actually consume that much gas, but it would be much safer to specify a more reasonable number. For a simple transfer between accounts, 21,000 should do. If you're sending ether to a contract that does some computation, consider using 100,000.

This is described in web3py Documentation

Once you have your provider set and web3 instantiates you can do:

signed_txn = w3.eth.account.signTransaction(dict(
    nonce=w3.eth.getTransactionCount('yourAddress'),
    gasPrice = w3.eth.gasPrice, 
    gas = 100000,
    to='recipientAddress',
    value=web3.toWei(12345,'ether')
  ),
  'yourprivatekey')

w3.eth.sendRawTransaction(signed_txn.rawTransaction)

It will give the hash of the transaction. If you have your keyfile but you do not know your privatekey you can get it using this tool included in web3py

with open('path to your keyfile') as keyfile:
    encrypted_key = keyfile.read()
    private_key = w3.eth.account.decrypt(encrypted_key, 'thepasswordforyour_keyfile')

Hope this helps.