I know about the Poison Mushroom and the difficulty of the sequel, but what is the main other difference?
The main difference between Super Mario Bros and The Lost Levels
super-mario-brossuper-mario-bros-the-lost-levels
Related Solutions
Here's a pretty comprehensive list which describes exactly how to reach each Warp Zone. There are actually quite a few (10 in total), many more than the first game.
From the screenshot shown on that page, it appears that the coin blocks have been relabeled with "25". I hope, at least, that this was the only "partially rearranged" issue in the 25th anniversary of a classic.
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Best Answer
Super mario bros: The lost levels was, in japan, Super Mario Bros 2;
But it was deemed as 'Too hard' for US audiences. So Nintendo took another game named Doki Doki Panic, reskinned it with Mario, and thats Mario 2 US.
The Japan Mario Bros 2 was then released in the US a few years later, but with Mario Bros 2 already a game, they named it The Lost Levels.
So Super Mario Bros: The lost levels is the direct sequel, with different levels and some different mechanics, to Super Mario Bros.
https://en.wikipedia.org/wiki/Super_Mario_Bros.:_The_Lost_Levels