I don't really know if it's from a game, but it surely looks like:
This animation is used as avatar in several different forums over the internet.
Which game is this animation of a guy with a green shirt, backpack, and blue pants from
game-identification
Related Topic
- Which Destiny-like FPS game is this picture from
- What game is this (looks like something related with House of Cards)
- Can someone recognize this game from a laptop commercial
- This game with the player character flying with purple wings
- Which game is shown in this image from apple website
- What game is this PS4 avatar soldier with a yellow helmet from
- Xbox – Which ‘Xbox One X enhanced’ game is this city from
Best Answer
That's the titular Boy, from A Boy and His Blob, on the NES.
Here he is alongside his Blob, I believe in the process of tossing the Blob a jellybean:
Animated GIF version of the toss on tumblr, for those interested